Semi-rational engineering of terminal deoxynucleotidyl transferase for high-efficiency enzymatic de novo DNA synthesis

Synthetic and Systems Biotechnology 2026 Open access

Read full text
Authors: Chengjie Zhang, Zhicong He, Zhengquan Gao, Chunxiao Meng, Wei Peng, Lei Du, Shengying Li
DOI: 10.1016/j.synbio.2026.03.009
Cited by: 3
References: 52

Abstract

The 21st century has witnessed rapid advancements in synthetic biology, with DNA synthesis emerging as a foundational technology. Conventional phosphoramidite-based methods face significant limitations, including short DNA elongation lengths (

Fields of Study & Subjects

CRISPR and Genetic Engineering DNA Repair Mechanisms DNA and Nucleic Acid Chemistry

Publication details

Journal / source
Synthetic and Systems Biotechnology
Publisher
Elsevier BV
Volume
13
Pages
519–530
Publication date
25 March 2026
Language
EN