Label-free, single-cell-precise, and monomeric-unit-resolved monitoring of biopolymer fermentation by ramanomics

Trends in biotechnology 2026 Open access

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Authors: Jia Zhang, Xi Liu, Zhihang Chen, X. Edward Zhou, Mingying Li, 孙增艳, Tao Xu, Taoran Zheng, Xiaomeng Wang, Xinnan Cui, Jiajun Du, Gongchao Jing
+8 more Yanmei Zhang, Mingqin Xu, Bo Ma, Fuli Li, Huiying Luo, Bin Yao, Guo-Qiang Chen, Jian Xu
DOI: 10.1016/j.tibtech.2026.03.017
Cited by: 1
References: 55

Abstract

Rapid yet high-information-content monitoring of cellular metabolism during fermentation is highly desirable for process optimization. While single-cell Raman spectroscopy can rapidly distinguish intracellular biopolymer classes, finer-resolution discrimination of monomeric units remains challenging. In this study, we demonstrated that the ramanome can classify polyhydroxyalkanoate (PHA)-producing Halomonas bluephagenesis cells that synthesize either polyhydroxybutyrate or poly(3-hydroxybutyrate-co-4-hydroxybutyrate) with 99.75% accuracy and simultaneously quantify total intracellular PHA and its constituent monomeric units (3-hydroxybutyrate and 4-hydroxybutyrate) at the single-cell level (median absolute deviation

Fields of Study & Subjects

Microbial Metabolic Engineering and Bioproduction Biopolymer Synthesis and Applications Fungal and yeast genetics research

Publication details

Journal / source
Trends in biotechnology
Publisher
Elsevier BV
Volume
44
Issue
9
Pages
2771–2795
Publication date
11 April 2026
Language
EN