Comparison of two DNA extraction methods for the detection of zoonotic protozoan pathogens in oysters and seawater

Veterinary and Animal Science 2026 Open access

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Authors: Veronica Rodriguez Fernandez, Minji Kim, Karen Shapiro
DOI: 10.1016/j.vas.2026.100592
Cited by: 2
References: 12

Abstract

We compared freeze-thaw and bead beating methods for extracting DNA from parasites in oysters and seawater. Bead beating performed better for extracting Cryptosporidium, Giardia , and Toxoplasma in seawater, while freeze-thaw yielded comparable or better detection for Cryptosporidium and Giardia in oysters. This comparison highlights relative strengths and limitations of these methods.

Fields of Study & Subjects

Parasitic Infections and Diagnostics Marine Bivalve and Aquaculture Studies Vibrio bacteria research studies

Publication details

Journal / source
Veterinary and Animal Science
Publisher
Elsevier BV
Volume
32
Pages
100592–100592
Publication date
7 February 2026
Language
EN