Optimized tRNA processing and TREX2–SpCas9 fusion enable high-efficiency multiplex genome editing in plants

Plant Communications 2026 Open access

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Authors: Ziyan Xu, Shengqun Qiu, Yuchong Tan, Yongjie Kuang, Yang, Chao, 1961-, Fang Yan, Xueping Zhou, Huanbin Zhou
DOI: 10.1016/j.xplc.2026.101921
Cited by: 1
References: 46

Abstract

elements, enabling highly efficient processing of multiplexed gRNA arrays. Incorporating these top-performing tRNAs into our system enabled simultaneous editing of up to 29 OsCPK genes in a single rice plant. Furthermore, we demonstrated the cross-species applicability of this platform in the dicot Nicotiana benthamiana using transient expression, where rice-derived tRNA elements facilitated high-efficiency editing. This optimized multiplex gene-editing system provides a robust, scalable platform for accelerating plant functional genomics and engineering complex agronomic traits.

Fields of Study & Subjects

CRISPR and Genetic Engineering RNA and protein synthesis mechanisms Chromosomal and Genetic Variations

Publication details

Journal / source
Plant Communications
Publisher
Elsevier BV
Volume
7
Issue
7
Pages
101921–101921
Publication date
20 May 2026
Language
EN