Protocol for minimizing off-target neuronal activation during optical stimulation in vivo
Abstract
In vivo optogenetic stimulation is a powerful approach for dissecting neuronal circuit function, yet it can inadvertently lead to activation of endogenous retinal opsins, producing off-target responses that propagate to the cortex and therefore confound experimental interpretation. Here, we present a protocol for optical device implantation in the mouse brain that minimizes light leakage from the implant and skull. We also describe in vivo electrophysiological techniques to assay off-target neuronal activity and ensure its amelioration. For complete details on the use and execution of this protocol, please refer to Weiler et al. 1
Fields of Study & Subjects
Photoreceptor and optogenetics research
Neuroscience and Neural Engineering
Advanced Fluorescence Microscopy Techniques
Publication details
- Journal / source
- STAR Protocols
- Publisher
- Elsevier BV
- Volume
- 7
- Issue
- 1
- Pages
- 104342–104342
- Publication date
- 19 January 2026
- Language
- EN