Protocol to study mRNA levels during endoreplication in Arabidopsis thaliana through nuclei extraction and a sorting strategy
Abstract
In plants, differentiation and expansion of some cell types is accompanied by exit from cell division and onset of endoreplication. Here, we describe a protocol to study changes in mRNA levels during endoreplication in Arabidopsis thaliana cotyledons. We provide detailed steps for the isolation of total nuclei and a strategy to sort stained nuclei according to their ploidy level. Finally, we detail procedures for RNA extraction, a housekeeping gene, and a marker gene for qPCR across ploidy.
Fields of Study & Subjects
Plant Molecular Biology Research
RNA Research and Splicing
Plant Reproductive Biology
Publication details
- Journal / source
- STAR Protocols
- Publisher
- Elsevier BV
- Volume
- 7
- Issue
- 1
- Pages
- 104350–104350
- Publication date
- 28 January 2026
- Language
- EN