Protocol for non-invasive delivery of CRISPR RNPs via virus-like particles for mouse model generation

STAR Protocols 2026 Open access

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Authors: Da Eun Yoon, Jiyun Yang, Tae Yeong Jeong, Jeongeun Park, Hyunji Lee, Je Kyung Seong, Kyoungmi Kim
DOI: 10.1016/j.xpro.2026.104352
Cited by: 2
References: 14

Abstract

CRISPR-virus-like particle (VLP)-induced targeted mutagenesis (CRISPR-VIM) enables genome editing in mouse embryos through non-invasive delivery of CRISPR ribonucleoproteins (RNPs) via VLPs, eliminating the need for physical manipulation and specialized expertise. We detail protocols for VLP production, titration, and treatment for diverse genome edits. This protocol is compatible with zygotes and in vitro fertilization (IVF)-derived embryos via simple co-culture, facilitating high-efficiency and heritable mutations with minimized off-target effects, independent of specialized equipment and conducive to reduced animal use. For complete details on the use and execution of this protocol, please refer to Jeong et al. 1

Fields of Study & Subjects

CRISPR and Genetic Engineering Pluripotent Stem Cells Research Virus-based gene therapy research

Publication details

Journal / source
STAR Protocols
Publisher
Elsevier BV
Volume
7
Issue
1
Pages
104352–104352
Publication date
4 February 2026
Language
EN