Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei

STAR Protocols 2026 Open access

Read full text
Authors: Binbin Cui, Xiayu Chen, Mingfang Wang, Yinyue Deng
DOI: 10.1016/j.xpro.2026.104353
Cited by: 0
References: 9

Abstract

Here, we present a protocol to detect the signaling network composed of cyclic AMP (cAMP) and cyclic di-GMP (c-di-GMP) signaling systems in Shigella sonnei . Technical assays include microscale thermophoresis (MST), quantitative reverse-transcription PCR (RT-qPCR), ultra-high-performance liquid chromatography-mass spectrometry (LC-MS), electrophoretic mobility shift assay (EMSA), bacterial adenylate cyclase two-hybrid (BACTH) assay, and molecular docking. For complete details on the use and execution of this protocol, please refer to Wang et al. 1

Fields of Study & Subjects

Bacterial Genetics and Biotechnology Bacterial biofilms and quorum sensing Escherichia coli research studies

Publication details

Journal / source
STAR Protocols
Volume
7
Issue
1
Pages
104353–104353
Publication date
10 February 2026
Language
EN