Protocol for intranuclear Nrf2 detection in activated mouse, human, and Jurkat T cells by spectral flow cytometry

STAR Protocols 2026 Open access

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Authors: Aprajita Tripathi, Debolina Dasgupta, Mehak Ahuja, Gabrielle Fisette, Nadine Santana-Magal, Kalyani Pyaram
DOI: 10.1016/j.xpro.2026.104360
Cited by: 1
References: 17

Abstract

Nuclear factor erythroid 2-related factor 2 (Nrf2) coordinates cellular antioxidation responses. Its expression increases in T cells upon activation. Here, we present a protocol for quantitative detection of intranuclear Nrf2 in activated mouse, human, and Jurkat T cells using spectral flow cytometry. We describe procedures for primary T cell isolation, in vitro activation, intranuclear staining, and flow cytometric acquisition and analysis, including gating strategies for CD4 + and CD8 + T cells. For complete details on the use and execution of this protocol, please refer to Tripathi et al. 1

Fields of Study & Subjects

Genomics phytochemicals and oxidative stress Skin Protection and Aging Single-cell and spatial transcriptomics

Publication details

Journal / source
STAR Protocols
Publisher
Elsevier BV
Volume
7
Issue
1
Pages
104360–104360
Publication date
11 February 2026
Language
EN