Protocol to study the contribution of biomechanics in osteoarthritis development using a cartilage-on-chip model

STAR Protocols 2026 Open access

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Authors: Elodie Faure, Véronique Chobaz, Diego de Haro, Elsa Lauwers, Nathalie Busso, Carlo Alberto Paggi, Sonia Nasi
DOI: 10.1016/j.xpro.2026.104365
Cited by: 0
References: 2

Abstract

Here, we present a protocol to study biomechanics and calcification in osteoarthritis using a cartilage-on-chip model. We describe steps for culturing human chondrocytes in 3D hydrogels and subjecting them to physiological loading. We then detail procedures for assessing spontaneous calcification via alizarin red S staining, gene expression using qPCR, protein expression by immunofluorescence, and protein secretome analysis through the Olink proximity extension assay. This protocol is adaptable and allows exploration of cartilage degeneration mechanisms under controlled conditions.

Fields of Study & Subjects

Osteoarthritis Treatment and Mechanisms 3D Printing in Biomedical Research Cellular Mechanics and Interactions

Publication details

Journal / source
STAR Protocols
Volume
7
Issue
1
Pages
104365–104365
Publication date
11 February 2026
Language
EN