Protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes

STAR Protocols 2026 Open access

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Authors: Ankur Kumar, Hua Bai
DOI: 10.1016/j.xpro.2026.104370
Cited by: 0
References: 15

Abstract

Mitochondrial dynamics are essential for cellular homeostasis and can be visualized in adult Drosophila oenocytes using live-cell confocal imaging. Here, we present a protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes. We describe steps for fly preparation, dissection of abdominal cuticle to expose oenocytes, and mounting. We then detail procedures for time-lapse acquisition of mitochondria labeled with mitoGFP. Optimized imaging parameters enable reproducible visualization of mitochondrial morphology stably for longer durations.

Fields of Study & Subjects

Mitochondrial Function and Pathology Autophagy in Disease and Therapy Advanced Fluorescence Microscopy Techniques

Publication details

Journal / source
STAR Protocols
Volume
7
Issue
1
Pages
104370–104370
Publication date
16 February 2026
Language
EN