Protocol for isolation of total RNA from mouse whole cochlea using organic solvents
Abstract
Determining gene expression changes in cochlear components is important for understanding the mechanisms underlying hearing loss. Here, we present a protocol for the isolation of total RNA from mouse cochleae across a wide range of ages (2–25 weeks old) using organic solvent extraction. We describe the steps for obtaining the entire cochlea from mice and isolating high-quality total RNA suitable for further reverse transcription and PCR. For complete details on the use and execution of this protocol, please refer to Rías et al. 1
Fields of Study & Subjects
Hearing
Cochlea
Tinnitus
Genetics
Molecular Biology Techniques and Applications
Hearing Loss and Rehabilitation
Publication details
- Journal / source
- STAR Protocols
- Volume
- 7
- Issue
- 1
- Pages
- 104376–104376
- Publication date
- 16 February 2026
- Language
- EN