Protocol for generating a 3D hydrogel-based tumor resection model in vitro using pancreatic cancer cells
Abstract
We present a protocol for the generation of two distinct in vitro tumor resection models to evaluate cellular responses at tumor margins in 3D. We describe steps for patterning pancreatic cancer cells (Panc-01) embedded in hydrogels using a custom 96-pin metal lid to create standardized resection cavities. In a second model, we detail procedures for refining the protocol to imitate narrow surgical resection margins. This protocol supports diverse treatment modalities and enables reproducible, high-throughput analysis of post-resection responses. For complete details on the use and execution of this protocol, please refer to Miebach et al. 1
Fields of Study & Subjects
3D Printing in Biomedical Research
Cancer Cells and Metastasis
Tissue Engineering and Regenerative Medicine
Publication details
- Journal / source
- STAR Protocols
- Publisher
- Elsevier BV
- Volume
- 7
- Issue
- 1
- Pages
- 104416–104416
- Publication date
- 1 March 2026
- Language
- EN