Protocol for visualizing microglial lysosomal content by immunohistochemistry in the rodent brain
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Microglia, the resident phagocytes of the central nervous system, clear diverse substrates in development, aging, injury, and disease. Here, we present a protocol to visualize phagocytosed content within microglial lysosomes in the rodent brain using immunohistochemistry and confocal microscopy. We describe the steps for tissue staining, image acquisition, and lysosome analysis. We then detail the procedures for microglia and lysosome reconstruction for 3D visualization. For complete details on the use and execution of this protocol, please refer to Gu et al. 1
Fields of Study & Subjects
Neuroinflammation and Neurodegeneration Mechanisms
Autophagy in Disease and Therapy
Phagocytosis and Immune Regulation
Publication details
- Journal / source
- STAR Protocols
- Volume
- 7
- Issue
- 2
- Pages
- 104438–104438
- Publication date
- 16 March 2026
- Language
- EN