Protocol for generating and characterizing Matrigel-free prostate cancer patient-derived organoids

STAR Protocols 2026 Open access

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Authors: Robin Dolgos, Romuald Parmentier, Jing Wang, Arnoud J. Templeton, Kirsten D. Mertz, Heike Pueschel, Helge Seifert, Ashkan Mortezavi, Tatjana Vlajnic, Cyrill A. Rentsch, Lukas Bubendorf, Clémentine Le Magnen
DOI: 10.1016/j.xpro.2026.104486
Cited by: 2
References: 6

Abstract

Here, we present a protocol for the generation of prostate cancer patient-derived organoids (PCa PDOs) in Matrigel-free conditions. We describe steps for sample selection, pathological resection, and tissue dissociation. We then detail procedures for the generation, maintenance, and characterization of the PCa PDOs. Finally, we provide guidance for the processing of PDOs for single-cell RNA sequencing (scRNA-seq) analysis and using the prostate PDO single-cell atlas (PPScA) for both novice and advanced bioinformatic users. For complete details on the use and execution of this protocol, please refer to Dolgos et al. 1

Fields of Study & Subjects

Cancer Cells and Metastasis Prostate Cancer Treatment and Research Extracellular vesicles in disease

Publication details

Journal / source
STAR Protocols
Publisher
Elsevier BV
Volume
7
Issue
2
Pages
104486–104486
Publication date
7 April 2026
Language
EN