Protocol for performing traumatic spinal cord injury surgery on mice in germ-free isolators and assessing injury recovery and pathophysiology
Abstract
An altered gut microbiome affects recovery from traumatic spinal cord injury (SCI). Here, we present a protocol for performing SCI survival surgery on mice in germ-free isolators. Specifically, we describe steps for colonization, preparing surgical tools, performing surgery, and post-surgical care. We then outline procedures for behavioral assessment, followed by collecting and processing tissue to assess injury recovery and pathophysiology. This protocol can be easily adapted to study the gut microbiome's influence on other forms of central nervous system injury.
Fields of Study & Subjects
Spinal Cord Injury Research
Nerve injury and regeneration
Nerve Injury and Rehabilitation
Publication details
- Journal / source
- STAR Protocols
- Publisher
- Elsevier BV
- Volume
- 7
- Issue
- 2
- Pages
- 104488–104488
- Publication date
- 7 April 2026
- Language
- EN