Protocol for deriving proliferating primary fibroblast cultures from Malabar red snapper larvae for cytogenetic analysis

STAR Protocols 2026 Open access

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Authors: Kathiresan Purushothaman, Muhammad Hazim bin Mohamed, Saraphina Dianne Tneo Rwei Qing, Nguyen Thanh Vu, Bing Liang, Harmeet Singh, Tan Shixiong, Grace Loo, Saraswathy Bisa, Dean R. Jerry, Jose A. Domingos, Shubha Vij
DOI: 10.1016/j.xpro.2026.104489
Cited by: 1
References: 17

Abstract

Here, we present a protocol for deriving primary fibroblast cultures from early-stage Malabar red snapper (Lutjanus malabaricus) larvae for cytogenetic analysis. We describe steps for dissociating larval tissues using trypsin and culturing them to establish proliferating fibroblast cells. We then detail procedures for arresting cells at metaphase by colchicine treatment, followed by hypotonic treatment, methanol-acetic acid fixation, and Giemsa staining to visualize chromosomes. This protocol yields clear metaphase spreads suitable for karyotype analysis and downstream cytogenetic applications.

Fields of Study & Subjects

Physiological and biochemical adaptations Molecular Biology Techniques and Applications Reproductive biology and impacts on aquatic species

Publication details

Journal / source
STAR Protocols
Publisher
Elsevier BV
Volume
7
Issue
2
Pages
104489–104489
Publication date
7 April 2026
Language
EN