Protocol for purifying and sequencing cell subpopulations based on RNA FISH signal in planaria

STAR Protocols 2026 Open access

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Authors: Mary Cathleen McKinney, Jason A. Morrison, Frederick G. Mann, Dung M. Vuu, Carolyn E. Brewster, Kate Hall, Blair W. Benham-Pyle, Alejandro Sánchez Alvarado
DOI: 10.1016/j.xpro.2026.104547
Cited by: 1
References: 9

Abstract

Isolation and characterization of rare cell types in non-model organisms present a challenge. Here, we provide a protocol to purify specific cells from planaria for downstream RNA sequencing. We employ a one-step dissociation and fixation method that avoids RNA-degrading formaldehyde. We label cells using RNA fluorescence in situ hybridization (RNA FISH) and flow sort to enrich sequencing reads from cells of interest for deeper biological insight. For complete details on the use and execution of this protocol, please refer to Mann et al. 1

Fields of Study & Subjects

Planarian Biology and Electrostimulation Protist diversity and phylogeny Marine Ecology and Invasive Species

Publication details

Journal / source
STAR Protocols
Publisher
Elsevier BV
Volume
7
Issue
2
Pages
104547–104547
Publication date
9 May 2026
Language
EN