Protocol for determining minimum inhibitory concentrations of essential oils against bacterial pathogens using broth microdilution

STAR Protocols 2026 Open access

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Authors: Maura Di Vito, Melinda Mariotti, Mattia Di Mercurio, Domiziana Coggiatti, Silvia Rizzo, Abdesselam Zhiri, Francesca Bugli, Maurizio Sanguinetti, Brunella Posteraro
DOI: 10.1016/j.xpro.2026.104587
Cited by: 1
References: 6

Abstract

Essential oils (EOs) are widely investigated for antimicrobial activity, yet minimum inhibitory concentrations (MICs) lack cross-study comparability due to the absence of a harmonized reference assay. Here, we present a protocol to determine MICs of EOs against aerobic bacterial pathogens using a broth microdilution technique. We describe steps for incorporating EO dispersion using Tween 80 and preparing a standardized bacterial inoculum. We detail procedures for establishing two-fold dilution series in 96-well plates and visually determining the MIC endpoint.

Fields of Study & Subjects

Essential Oils and Antimicrobial Activity Nigella sativa pharmacological applications Insect Pest Control Strategies

Publication details

Journal / source
STAR Protocols
Publisher
Elsevier BV
Volume
7
Issue
2
Pages
104587–104587
Publication date
21 May 2026
Language
EN