Protocol for monitoring intestinal plasma membrane integrity breached by bacterial pore-forming toxin in Caenorhabditis elegans
Abstract
Plasma membrane integrity (PMI) safeguards cellular homeostasis but can be disrupted by bacterial pore-forming toxins (PFTs), necessitating complete pore repair for host survival. Here, we present an in vivo protocol for monitoring apical plasma membrane perforation and its restoration in intestinal epithelial cells of C. elegans following intoxication with the PFT Cry5B. We describe steps for utilizing membrane-impermeable dyes, such as propidium iodide (PI), as a functional readout to assess PMI and quantitatively evaluate pore-repair capacity in C. elegans . For complete details on the use and execution of this protocol, please refer to Hsieh, HC et al. 1
Fields of Study & Subjects
Genetics
Aging
and Longevity in Model Organisms
Cellular transport and secretion
Endoplasmic Reticulum Stress and Disease
Publication details
- Journal / source
- STAR Protocols
- Publisher
- Elsevier BV
- Volume
- 7
- Issue
- 3
- Pages
- 104679–104679
- Publication date
- 1 July 2026
- Language
- EN