RXFP2 gene variation drives horn sexual differences and polledness in sheep

This study performed deep sequencing of the RXFP2 gene in three sheep breeds: PT, QHS, and HS. Association analysis identified four significant variant loci: SNP25, SNP57, SNP70, and SNP28. GMDR and LASSO analyses confirmed the crucial role of SNP28 in horn trait regulation. Loci interaction analysis revealed an epistatic effect between SNP25 and SNP57, moreover further supporting that SNP25 may be a locus contributing to breed-specific differentiation. Linkage disequilibrium analysis indicated that SNP70 exhibits stable heritability, while the genetic effect of SNP57 is influenced by its haplotype background. Population genetics validation demonstrated that SNP28 and SNP57 contribute to larger horn size in sheep, with SNP57 exhibiting a sex-specific effect. SNP70 showed strong explanatory power for the polled trait in Plateau-type Tibetan sheep but may be associated with the pathogenicity of cryptorchidism, while SNP25 was confirmed to be unrelated to horn traits. Furthermore, preliminary genetic studies on the scurs trait revealed its underlying genetic complexity. These findings provide promising genetic tools for sheep breeding.