Dietary cobalt suppresses starch-induced succinate accumulation via colonic synthesis of vitamin B 12 and its analogues in rats

Abstract High-amylose maize starch (HAMS) can lead to succinate accumulation in the rat colon depending on the colonic microbiota. Since succinate is primarily produced via the vitamin B 12 (VB 12 )-dependent succinate pathway, limited VB 12 availability in the colon may impair fermentation. While a portion of dietary VB 12 may reach the colon, most of it is absorbed in the upper gastrointestinal tract, potentially resulting in an insufficient supply for colonic bacteria. This study aimed to determine the minimum caecal VB 12 concentration required to prevent succinate accumulation and to assess whether dietary cobalt (Co), a structural component of VB 12 and its analogues, promotes microbial VB 12 analogue synthesis. Sprague-Dawley male rats were used in three experiments. In Experiment 1, HAMS-fed rats were given diets with increasing VB 12 doses. Caecal succinate concentrations decreased dose-dependently, with a predicted threshold of 74 pmol/g VB 12 required to prevent accumulation. In Experiment 2, rats were fed HAMS diets with varying Co levels. Co supplementation significantly increased VB 12 -equivalent concentrations, measured by microbiological assay, from 27 to 915 pmol/g without altering cobalamin concentrations, suggesting enhanced microbial synthesis of VB 12 analogues. Caecal succinate levels decreased with increasing Co intake, mimicking the effects of dietary VB 12 . In Experiment 3, rats were fed HAMS diets with or without high-dose Co to confirm these effects and assess microbiota changes. Co supplementation restored the abundance of Akkermansia , which utilises VB 12 and its analogues. These findings suggest that maintaining sufficient colonic VB 12 – through direct supplementation or Co-stimulated microbial production – may help mitigate HAMS-induced succinate accumulation and support balanced colonic fermentation.