Epitope-Directed Antibody Development and Enhancement for Broad-Spectrum Fentanyl-Class Substance Detection: From Rational Hapten Design to On-Site Analysis

Analytical Chemistry 2026

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Authors: Xiaoyi Chen, Xiang Chi, Yuxi Hu, Xinyi Gao, Xin Wang, Yawen Shu, Daichun Wei, Peng Xu, Bingjie Zou, Bin Di
DOI: 10.1021/acs.analchem.5c08177
Cited by: 1
References: 52

Abstract

value = 0.072-101.7 ng/mL). Furthermore, a rapid gold nanoparticle-based immunochromatographic assay (GICA) with a cutoff value of 5 ng/mL was established and demonstrated its utility as a robust on-site tool. Both assays showed excellent accuracy in spiked human urine and hair samples, with recovery rates of 80.20-118.65%. This work provides not only a powerful tool for the surveillance of fentanyl analogues in complex biological matrices but also a rational hapten design framework for generating antibodies with tunable properties.

Fields of Study & Subjects

Monoclonal and Polyclonal Antibodies Research Biosensors and Analytical Detection Pharmacogenetics and Drug Metabolism

Publication details

Journal / source
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Volume
98
Issue
17
Pages
12560–12573
Publication date
18 April 2026
Language
EN