Integrating Acridinium Ester with a Soluble Macromolecular Amplification Carrier for Ultrasensitive Detection of Aβ1-42 in Plasma
Abstract
The research presents the development of a novel ultrasensitive immunoassay for the detection of amyloid-β 1-42 (Aβ1-42) in plasma, a key biomarker for Alzheimer’s disease, through the integration of a soluble signal amplification platform (Ficoll400-SA) with an acridinium ester (AE) direct chemiluminescence system. The synthesis of Ficoll400-SA was optimized to maximize the loading of AE molecules by adjusting cross-linker activation ratios and AE labeling density, achieving an approximately 35-fold enhancement of signal intensity relative to conventional streptavidin-AE conjugates. The immunoassay protocol was further refined to improve sensitivity and specificity, resulting in a limit of detection (LoD) of 0.15 pg·mL –1 and a lower limit of quantitation (LLoQ) of 0.31 pg·mL –1 . The assay exhibited excellent specificity, with negligible cross-reactivity (
Fields of Study & Subjects
Alzheimer's disease research and treatments
Cholinesterase and Neurodegenerative Diseases
Protein Interaction Studies and Fluorescence Analysis
Publication details
- Journal / source
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Volume
- 98
- Issue
- 18
- Pages
- 13587–13597
- Publication date
- 28 April 2026
- Language
- EN