Mass Spectrometry Imaging of Glycosphingolipid C═C Position Isomers by On-tissue Capture-Epoxidation on a Diselenide-Functionalized Covalent Organic Framework
Abstract
onto slides for tissue thaw-mounting, on-tissue capture-epoxidation was completed within 5 min. With a two-step ambient liquid extraction MS/MS imaging workflow, epoxidized phospholipids and glycosphingolipids on tissue were imaged separately in each step. Compared with the conventional method, this strategy substantially improved the lipid coverage of isomer-differentiated MSI by 7-fold in brain tissue, especially for glycolipids or sphingolipids (27 vs 0). This work illuminates the inaccessible spatial landscape of glycosphingolipid C═C isomers, opening new avenues for understanding their roles in brain function and disease.
Fields of Study & Subjects
Polydiacetylene-based materials and applications
Supramolecular Self-Assembly in Materials
Mass Spectrometry Techniques and Applications
Publication details
- Journal / source
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Volume
- 98
- Issue
- 18
- Pages
- 13676–13687
- Publication date
- 29 April 2026
- Language
- EN