Catalytic and Gating Nanoreactors: Cu(I)/Cu(II)-MOF@HMS for Size-Selective DNA-Templated Click Ligation Chain Reaction and Detection of Nucleic Acids
Abstract
for 50 nt analogues. Consequently, the 4 nm nanoreactors enable exponential signal amplification for 20 nt templates, achieving an amplification efficiency of 69% and a limit of detection of 57.5 aM, while effectively suppressing the signal from 30 to 50 nt analogues. To validate the specificity for biological targets, this mechanism affords over 20-fold discrimination of miRNA-21 against its longer pre-miRNA-21 precursor, confirming that the hydrodynamic diameter mismatch effectively resolves the fundamental challenge of nested sequence interference.
Fields of Study & Subjects
Advanced biosensing and bioanalysis techniques
Metal-Organic Frameworks: Synthesis and Applications
Advanced Nanomaterials in Catalysis
Publication details
- Journal / source
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Volume
- 98
- Issue
- 18
- Pages
- 13883–13896
- Publication date
- 29 April 2026
- Language
- EN