Deep-UV Resonance Raman Spectroscopy Enables Direct Quantification of mRNA in Intact Lipid Nanoparticle Formulations

Analytical Chemistry 2026

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Authors: Kathleen Korson Sullivan, Kathryn Wright, Angie Kim, Jamie Gilmore, Jake Dexter, Russell Umstead, Ray Reid, Rohit Bhartia, Ziad Ganim, Mohindra Seepersaud
DOI: 10.1021/acs.analchem.6c02624
Cited by: 1
References: 18

Abstract

Deep-UV resonance Raman spectroscopy (DUV-RRS) enables rapid, label-free quantification of mRNA directly in intact lipid nanoparticles. Without deformulation, seconds-scale acquisitions isolate adenine and guanine signatures with minimal lipid/water interference, yielding calibrated concentration estimates across buffers and formulations. A multipixel SNR strategy improves detection at low signal. This in situ assay offers a high-throughput readout for manufacturing and quality control of mRNA formulations.

Fields of Study & Subjects

Spectroscopy Techniques in Biomedical and Chemical Research RNA Interference and Gene Delivery Mass Spectrometry Techniques and Applications

Publication details

Journal / source
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Volume
98
Issue
22
Pages
16704–16707
Publication date
27 May 2026
Language
EN