Acoustic Droplet Ejection High-Resolution Mass Spectrometry Enables High-Throughput Analysis of Proteins in Complex Biologic and Vaccine Matrices

Accelerating the discovery and development of life-saving therapeutics and vaccines requires a commensurate level of analytical innovations, especially high-throughput (HT) methodologies for simultaneous multicomponent analyses traditionally used in fast-paced laboratory settings. HT screening is a critical step to enable lead candidate design, selection, and formulation at aggressive timelines. Current methods used for this purpose, such as liquid chromatography-mass spectrometry (LC-MS)-based approaches, are intrinsically low-throughput. Such assays are less meaningful in settings where processing thousands of samples is required to guide critical decisions quickly. Acoustic droplet ejection-open-port interface-mass spectrometry (ADE-OPI-MS) is a cutting-edge technology that eliminates the need for LC by introducing nanoliter-scale samples to an MS via acoustic ejection at speeds of