A Self-Cross-Linked Dual-Enzyme Assembly for Safe and Efficient Hyperuricemia Therapy
Abstract
-degrading enzyme, via engineered coiled-coil motifs. Complementary motifs bearing site-specific cysteine residues were designed to promote disulfide-stabilized heterodimerization, and their binding specificity and affinity were validated using a fluorescence-based assay. These motifs were then fused to UOX and CAT to enable spontaneous and stable assembly of a functional enzyme complex without chemical conjugation. The resulting complex exhibited high catalytic efficiency and significantly reduced reactive oxygen species-mediated cytotoxicity in human colon carcinoma cells. This work provides a tunable strategy for dual-enzyme assembly, enhancing therapeutic synergy while minimizing oxidative side effects in hyperuricemia treatment.
Fields of Study & Subjects
Gout
Hyperuricemia
Uric Acid
Biochemical and Molecular Research
Supramolecular Self-Assembly in Materials
Publication details
- Journal / source
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Volume
- 27
- Issue
- 2
- Pages
- 1210–1222
- Publication date
- 23 January 2026
- Language
- EN