Hemolytik 2: An Updated Database of Hemolytic Peptides and Proteins

Hemolytik 2.0 (http://webs.iiitd.edu.in/raghava/hemolytik2/) is a comprehensive, manually curated database that provides experimentally validated information on both hemolytic and nonhemolytic peptides. Data were meticulously extracted from peer-reviewed publications and established peptide repositories, including the Antimicrobial Peptide Database, UniProt, the Collection of Antimicrobial Peptides (CAMP-R4), and the data repository of antimicrobial peptides (DRAMP 4.0). This updated version of the original Hemolytik resource comprises 13,215 unique entries from 1645 research articles, representing approximately 7534 unique peptides. Each entry in Hemolytik 2.0 offers detailed annotations, including peptide name, amino acid sequence, biological source and origin, functional characterization, terminal modifications, stereochemistry, structural classification (linear or cyclic), and experimentally determined hemolytic activity. In addition, the database provides molecular representations of peptides in SMILES (Simplified Molecular Input Line Entry System) format, alongside predicted tertiary structures and annotated secondary structural states. Additionally, a RESTful API has been integrated into the Hemolytik 2.0 repository to enable programmatic access and automated retrieval of peptide data. Hemolytik 2.0 serves as a valuable resource for the scientific community, particularly for researchers involved in the design and development of therapeutic peptides, by facilitating the identification and optimization of peptide candidates with minimal hemolytic potential and enhanced safety profiles. In addition, Hemolytik 2.0 is also available on GitHub (https://github.com/raghavagps/Hemolytik2), where users can download the complete systematic data in different formats.