Sensitive Quantification of the Ethylene Oxide Hemoglobin Adduct N -(2-Hydroxyethyl)- l -valine from Minimal Blood Volumes

Chemical Research in Toxicology 2026

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Authors: Chih‐Wei Liu, Jiahao Feng, Jingya Peng, Haoduo Zhao, Xueying Wang, B. Bhaskar Gollapudi, Abby A. Li, James E. Bus, Christopher Kirman, Kun Lu
DOI: 10.1021/acs.chemrestox.6c00200
Cited by: 1
References: 7

Abstract

-(2-hydroxyethyl)-l-valine (HE-V). However, current methods require large blood volumes, limiting small-animal exposure studies and reliable detection of endogenous background levels of HE-V. We optimized a highly sensitive analytical workflow including hemoglobin extraction from microliter-scale blood samples, HE-V purification, and targeted LC-MS/MS quantitative analysis. This workflow reliably quantifies endogenous HE-V levels of unexposed mice from only 50 μg of purified hemoglobin (10 μL of blood) and is demonstrated to detect significant HE-V increases at EtO exposures as low as 0.05 ppm. Our platform enables practical EtO biomonitoring in volume-constrained experimental and environmental settings.

Fields of Study & Subjects

Hemoglobin structure and function Carcinogens and Genotoxicity Assessment Hemoglobinopathies and Related Disorders

Publication details

Journal / source
Chemical Research in Toxicology
Publisher
American Chemical Society (ACS)
Volume
39
Issue
6
Pages
1084–1087
Publication date
23 May 2026
Language
EN