The Transcription Factor AaMYB16 Regulates Freezing Stress in Actinidia Arguta Siebold & Zucc. Planch. ex Miq

Low temperature has a serious effect on Actinidia arguta growth and development. However, the mechanisms of how MYB proteins participate in freezing stress in A. arguta are still unclear. In this study, AaMYB16 was transformed into Arabidopsis thaliana and A. arguta . The relevant physiological and biochemical indexes were determined, and the regulatory model of AaMYB16 was preliminarily discussed. The results showed that there was an interaction between AaMYB16 and AabHLH137. AabHLH18 and AabHLH137 played a role in the upstream of AaMYB16 . Overexpress AaMYB16 increased plant susceptibility to freezing stress, whereas silencing AaMYB16 enhanced freezing tolerance in A. arguta . Our results suggest that AaMYB16 is a negative regulator of freeze response in A. arguta . The AabHLH18/AabHLH137-AaMYB16 module plays an important role in freezing stress. These results provide insights into the molecular mechanism of low-temperature regulation of A. arguta, and a theoretical basis for fruit storage and breeding of A. arguta .