Computational-Aided Mutagenesis of Lipase CRL1 with Promoted Stability and Activity for Biosynthesis of Vitamin E Succinate
Abstract
) showed a 6.8-fold improvement over that of WT. Mutant 6M could generate vitamin E succinate with an improved yield of 77.8% (60 °C, 12 h). The synergistically functional modifications of the lipase pave the way for the mutated lipase to become a highly promising and robust biocatalyst for industrial applications.
Fields of Study & Subjects
Antioxidant Activity and Oxidative Stress
Enzyme Catalysis and Immobilization
Diet
Metabolism
and Disease
Publication details
- Journal / source
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Volume
- 74
- Issue
- 20
- Pages
- 15727–15736
- Publication date
- 12 May 2026
- Language
- EN