Multilevel Residual Complexity Analysis Reveals Origin of Nanomolar Antiviral Bioactives of “Isoquercitrin”
Abstract
High Resolution Image Download MS PowerPoint Slide The remarkable reported in vitro and in vivo antiviral activity of a commercial, naturally derived, isoquercitrin sample (IQC90) against Ebola (EBOV), Zika virus (ZIKV), and SARS-CoV-2 could not be confirmed with a greater purity isoquercitrin (IQC). To resolve this discrepancy, IQC90 was subjected to a two-step, quantitative bioassay-guided fractionation employing countercurrent separation and gel filtration monitored by inhibition of syncytium formation in HEK293 cells transfected with SARS-CoV-2 spike protein and ACE2. This process revealed the IQC90 antiviral activity to be due to a new family of 21-hydroxyoleanane-3- O -oligosaccharides, named dicitriosides, present at
Fields of Study & Subjects
Carbohydrate Chemistry and Synthesis
Microbial Natural Products and Biosynthesis
Lipid Membrane Structure and Behavior
Publication details
- Journal / source
- Journal of Natural Products
- Publisher
- American Chemical Society (ACS)
- Volume
- 89
- Issue
- 4
- Pages
- 1249–1264
- Publication date
- 10 April 2026
- Language
- EN