Phomazaphilonoids A and B, Anti-inflammatory Polyketides from the Fungus Phomopsis sp. SCNU-F0049
Abstract
Phomazaphilones A and B ( 1 and 2 ), azaphilones possessing a 5/6/6-fused tricyclic ring system, along with two known analogues, were discovered from the fungus Phomopsis sp. SCNU-F0049 was collected from the mangrove sediment. The structures were elucidated by extensive spectroscopic data and single-crystal X-ray diffraction. Phomazaphilone A ( 1 ) possesses a new 6,7,8,8 a -tetrahydro-2 H -furo[4,3,2-de]isoquinolin-2-one core, and phomazaphilone B ( 2 ) possesses a new 6,7,8,8 a -tetrahydro-2 H,5 H -furo[4,3,2-de]isochromene-2,5-dione heterocyclic core. A possible biosynthetic pathway was proposed based on genome sequence analysis. Phomazaphilone B ( 2 ) inhibited NO production in LPS-stimulated RAW264.7 cells with an IC 50 value of 3.28 ± 0.24 μM and suppressed the expression levels of proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner without cytotoxicity.
Fields of Study & Subjects
Microbial Natural Products and Biosynthesis
Synthetic Organic Chemistry Methods
Fungal Biology and Applications
Publication details
- Journal / source
- Journal of Natural Products
- Publisher
- American Chemical Society (ACS)
- Volume
- 89
- Issue
- 4
- Pages
- 1309–1317
- Publication date
- 23 March 2026
- Language
- EN