Genome Mining Guided Identification of the Metallophore Delftichelin A from Delftia deserti

High Resolution Image Download MS PowerPoint Slide A targeted sequencing and genome mining approach for delftibactin-like biosynthetic pathways revealed three distinct biosynthetic gene cluster architectures (BGC del, dlc and dlp ) encoded in genomes of members of the genus Delftia . Comparative metabolomic analysis guided the isolation and characterization of a yet unreported metallophore, delftichelin A from Delftia deserti DSM1621 (previously named Delftia acidovorans DSM1621). Prediction of BGC architecture and A domain specificity was in line with the structure analysis uncovering previously unreported differences in amino acid composition and modifications. Analysis of bioactivity and metal-binding characteristics demonstrated that delftichelin A shows a preferential affinity for ferric iron, while also exhibiting heavy metal detoxification mechanisms via oxidative degradation, analogous to those reported for the delftibactin family of compounds.