Synergistic CRISPR–Cas9 Host Engineering and Enzyme Evolution for Enantioselective Synthesis of a Vibegron Pyrrolidine Intermediate
Summary
No summary yet. VIRAT can read this paper and write a plain-language summary of its aim, methods, key findings and limitations.
Summarise with VIRAT Free to try with your Science Catalogs account.Abstract
The stereoselective synthesis of chiral pyrrolidine motifs is essential to vibegron production but remains challenging using conventional chemical routes. Here we report an imine reductase (IRED) catalyzed asymmetric imine reduction to access a key vibegron intermediate. Directed evolution afforded a highly efficient variant delivering 94% conversion and >99% d.e. Combined enzyme and host engineering enabled clean whole cell catalysis, establishing a robust and scalable biocatalytic platform.
Fields of Study & Subjects
Enzyme Catalysis and Immobilization
Asymmetric Hydrogenation and Catalysis
Asymmetric Synthesis and Catalysis
Publication details
- Journal / source
- Organic Letters
- Volume
- 28
- Issue
- 12
- Pages
- 3695–3700
- Publication date
- 16 March 2026
- Language
- EN