A Single Residue Switch Controls Isomer Selectivity in the Myxobacterial Isobonnadiene Synthase
Abstract
Myxobacterial terpenoids are rare and only a few myxobacterial terpene synthases are known. A terpene synthase, IbdS, from Polyangium aurulentum sp. SDU3-1 was found to produce the [delta]9,10 isomer (1) of the spirocyclic 6/7/5-tricyclic bonnadiene, along with several 6/10-eunicellanes and cembrenoids as minor products. Sequence comparison and site-directed mutagenesis of IbdS and the actinobacterial bonnadiene synthase revealed a single residue switch at position 57 controlling isomer selectivity.
Fields of Study & Subjects
Plant biochemistry and biosynthesis
Microbial Natural Products and Biosynthesis
Sesquiterpenes and Asteraceae Studies
Publication details
- Journal / source
- Organic Letters
- Volume
- 28
- Issue
- 7
- Pages
- 2432–2436
- Publication date
- 3 February 2026
- Language
- EN