Discovery of Antibacterial Tetracycline-Type Natural Products Misiomycins E–H through KASIII Genome Mining and Regulator Overexpression

Organic Letters 2026

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Authors: Wen Fei Tan, Jing Xin Zhang, Jia Lin Sun, Ao Zhu, Jing Shi, Rui Hua Jiao, Zhi Kai Guo
DOI: 10.1021/acs.orglett.6c01650
Cited by: 1
References: 22

Abstract

Guided by KASIII genome mining in combination with an in situ gene cluster activation strategy, we discovered a series of tetracycline natural products featuring atypical starter units, misiomycins E–H ( 1–4, respectively), from Streptomyces misionensis CGMCC 4.1982. The structures were determined by HR-ESI-MS, 1D and 2D NMR spectroscopy, MS/MS fragmentation, and genomic data. Misiomycins G and H demonstrated potent antibacterial activity against Staphylococcus aureus, with minimum inhibitory concentrations (MICs) of 3.74 and 7.03 μg/mL, respectively.

Fields of Study & Subjects

Microbial Natural Products and Biosynthesis Synthesis and Biological Activity Microbial Metabolism and Applications

Publication details

Journal / source
Organic Letters
Publisher
American Chemical Society (ACS)
Volume
28
Issue
23
Pages
7196–7201
Publication date
29 May 2026
Language
EN