Nano-Enabled Broadband Near-Infrared Light Boosts Secondary Metabolite Production for Nanoliposomal Delivery toward Glioblastoma Applications

High Resolution Image Download MS PowerPoint Slide Ursolic acid (UA) and rosmarinic acid (RA) are secondary metabolites obtained from Mesona procumbens Hemsl. and exhibit potent antitumor properties. Using Gd 3 (Sc,Al) 2 Ga 3 O 12:Cr 3+ near-infrared phosphor-converted light-emitting diodes (NIR pc-LEDs), this study optimized UA and RA production by 16 and 48%, respectively. Liposomal encapsulation generated a nanoscale delivery system (RA/UA@LP, 290 ± 14 nm), improving their dispersity and establishing a nanoliposomal platform with in vitro BBB-crossing potential for brain-targeted delivery. Experiments revealed that in vitro, RA/UA@LP reduced U87MG glioblastoma cell viability to 49%, with no toxicity in SVGp12 cells. In vivo analysis using a heterotopic glioblastoma model (subcutaneous transplantation) showed that RA/UA@LP decreased tumor fluorescence radiance by 78% after 5 weeks, outperforming nonencapsulated RA/UA by 71%. This model facilitates noninvasive tumor monitoring while maintaining clinical relevance. The integration of NIR pc-LEDs and nanocarrier technology enhances therapeutic compound production and efficacy, advancing agriculture and oncology.