Optically Detected Magnetic Resonance Based Intracellular Thermometry Using Nanodiamonds Implanted in Adherent Cancer Cells

ACS Applied Nano Materials 2026 Open access

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Authors: John Consiglio, Rostislav Boltyanskiy, Yuliya Mindarava, Christian Laube, Wolfgang Knolle, Marjan Berishaj, Fedor Jelezko, Kayvan R. Keshari
DOI: 10.1021/acsanm.5c05655
Cited by: 3
References: 30

Abstract

Intracellular temperature influences cell metabolism and the tumor microenvironment, but there is a lack of consensus on basic thermal properties of cells and a shortage of reliable measurement tools. We utilized nanodiamonds (NDs) containing fluorescent Nitrogen Vacancy centers (NVs) implanted in glioblastoma cells to measure intracellular temperature via Optically Detected Magnetic Resonance (ODMR) and used dual fluorescence and bright-field imaging to establish ND-mitochondria distances. Careful ND selection, improved curve fitting, and time averaging enable repeatable measurement of cellular responses. Upon mitochondrial uncoupling with 5 μM FCCP, we reproducibly observed ODMR shifts consistent with an ∼1.7 °C temperature increase.

Fields of Study & Subjects

Diamond and Carbon-based Materials Research Mechanical and Optical Resonators thermodynamics and calorimetric analyses

Publication details

Journal / source
ACS Applied Nano Materials
Publisher
American Chemical Society (ACS)
Volume
9
Issue
8
Pages
3590–3596
Publication date
15 February 2026
Language
EN