Alcohol Oxidase–Imine Reductase Cascade for One-Pot Chiral Amine Synthesis

ACS Catalysis 2026 Open access

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Authors: Anya Miletic, Christopher J. Truby, Nicholas J. Turner, Rebecca E. Ruscoe
DOI: 10.1021/acscatal.5c06313
Cited by: 3
References: 21

Abstract

We report a one-pot biocatalytic cascade for the conversion of allylic alcohols into enantioenriched secondary amines through a sequence involving oxidation followed by conjugate reduction-reductive amination (CR-RA). This transformation, catalyzed by a cholesterol oxidase (ShCO) and an ene-imine reductase (EneIRED), proceeds under mild conditions and accommodates a broad substrate scope. In several cases, the cascade outperforms the stepwise method and exhibits substrate-dependent selectivity, highlighting the advantages of tandem enzymatic systems for efficient and streamlined chiral amine synthesis.

Fields of Study & Subjects

Enzyme Catalysis and Immobilization Cyclopropane Reaction Mechanisms Steroid Chemistry and Biochemistry

Publication details

Journal / source
ACS Catalysis
Publisher
American Chemical Society (ACS)
Volume
16
Issue
3
Pages
2119–2123
Publication date
14 January 2026
Language
EN