A Sonopiezoelectric Z-Scheme Nanoplatform Synergizing Trace Ag + for Eradicating Methicillin-Resistant Staphylococcus aureus -Induced Pneumonia

The underlying mechanisms by which heterogeneous interfaces enhance piezoelectric catalytic effects under ultrasonic irradiation, along with their potential biomedical applications, remain poorly understood. In this study, a versatile Z-Scheme nanoplatform by encapsulating the BiO 2-x /Ag 3 PO 4 heterojunction within poly(lactic- co -glycolic acid) (PLGA) microspheres (BA@PLGA) was developed for ultrasound-activated deep-lung antibacterial therapy. The Z-scheme heterojunction synergistically integrates piezoelectric and built-in electric fields, achieving 2.2 and 4.7-fold enhancements in piezoelectric ( d 33 ) and electromechanical coupling ( k ) coefficients, respectively. Ultrasonic-induced dynamic modulation of the piezoelectric potential in BiO 2-x /Ag 3 PO 4 generated a “carrier-pumping” effect, which enhanced polarized charge migration efficiency and thereby markedly amplified reactive oxygen species (ROS) production. Consequently, with the assistance of trace Ag +, 99.87 ± 0.05% of methicillin-resistant Staphylococcus aureus (MRSA) were effectively eradicated within 15 min in vitro . Prokaryotic transcriptome analysis further revealed that the synergistic effect of ROS and trace Ag + disrupted the cell envelope and interfered with the core metabolic pathways of MRSA. This ultrasound-activated platform, integrating piezocatalysis and trace Ag +, achieved over 99.9% bacterial clearance rapidly (1.6log superiority over gentamicin) and alleviated inflammation in a MRSA-induced pneumonia, demonstrating considerable potential for combating bacterial infections in deep-seated tissues.