Trans-Endothelial Trafficking in Zebrafish: Nanobio Interactions of Polyethylene Glycol-Based Nanoparticles in Live Vasculature

ACS Nano 2026

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Authors: Ye‐Wheen Lim, Craig A. Bell, Nicholas L. Fletcher, Nicholas D. Condon, Dewan Taslima Akhter, James Rae, Charles Ferguson, Nick Martel, James Humphries, Harriet P. Lo, Yeping Wu, Zachary H. Houston
+4 more Anne K. Lagendijk, Thomas E. Hall, Kristofer J. Thurecht, Robert G. Parton
DOI: 10.1021/acsnano.5c21042
Cited by: 2
References: 129

Abstract

Trans-endothelial transport of nanoparticles remains poorly characterized in live organisms. The zebrafish is a well-established model for direct in vivo imaging; however, standardized controls have not been consistently applied across studies. Here, we developed a standardized protocol to assess nanoparticle trans-endothelial trafficking in live zebrafish. We identified 2000 kDa dextran as an optimal coinjection control for standardizing microinjections and quantifying nanoparticle transport in a systematic, unbiased manner. Using the standardized protocol, we profiled early physiological trans-endothelial transport pathways in zebrafish embryos using dextran solutes as extravasation markers, showing that steady-state solute extravasation is characterized by paracellular routes selective for solutes

Fields of Study & Subjects

Caveolin-1 and cellular processes Cell Adhesion Molecules Research Zebrafish Biomedical Research Applications

Publication details

Journal / source
ACS Nano
Publisher
American Chemical Society (ACS)
Volume
20
Issue
3
Pages
3132–3155
Publication date
14 January 2026
Language
EN