Synthetic Biology Strategies for Activating Cryptic BGCs in Streptomyces : Engineering Native and Synthetic Promoters for Antibiotic Discovery
Abstract
has emerged as a powerful strategy to uncover novel antibiotics. Recent advances in native and synthetic promoter engineering have enabled precise transcriptional control over silent gene clusters. This review presents a comprehensive overview of constitutive and inducible promoters, both native and synthetic, used in refactoring efforts. It also highlights CRISPR-based activation systems and machine-learning-guided promoter design for BGC activation. Case studies illustrate how these strategies are translated into enhanced metabolite production, particularly in the context of antimicrobial resistance. This review aims to provide a systems-level understanding of promoter tools and their translational implications for natural product discovery.
Fields of Study & Subjects
Microbial Natural Products and Biosynthesis
Cyclopropane Reaction Mechanisms
Plant biochemistry and biosynthesis
Publication details
- Journal / source
- ACS Omega
- Publisher
- American Chemical Society (ACS)
- Volume
- 11
- Issue
- 1
- Pages
- 239–259
- Publication date
- 2 January 2026
- Language
- EN