Structure-Dynamics-Property Relationships in Silk Fibroin Films Probed via Solid-State NMR

High Resolution Image Download MS PowerPoint Slide Silk fibroin films offer tunable mechanical properties, yet the structural origins of this tunability are incompletely understood. Here, we show how solvent choice and post-treatment govern the mechanics of silk fibroin films by combining solid-state NMR relaxation measurements ( 13 C T 1 / T 1ρ; 1 H T 1 / T 1ρ ), X-ray diffraction, and TG-DTA analyses. Formic acid-derived films exhibited a 6-fold higher Silk II content and greater backbone constraint (longer 13 C T 1ρ ) than water-derived films, resulting in higher initial tensile strength. Methanol treatment had opposite effects on mechanical strength in formic acid-derived and water-derived films. In the former, it relaxed backbone constraints (shorter 13 C T 1ρ ) without changing the Silk II content, reducing strength. In the latter, it created nanoscale Silk II domains (