Palladium-Catalyzed Synthesis of 5-Formyl-2′-Deoxycytidine, 5-Carboxyl-2′-Deoxycytidine, and Nitrogen-15-Labeled Derivatives via Ex Situ-Generated Carbon Monoxide from Chloroform and Application in Isotope Dilution LC–MS/MS Analysis of DNA

High Resolution Image Download MS PowerPoint Slide Herein, we report a modular palladium-catalyzed carbonylative synthesis of unlabeled and 15 N 3 -labeled epigenetic DNA lesions 5-formyl-2′-deoxycytidine (5fdC) and 5-carboxyl-2′-deoxycytidine (5cadC) via ex situ CO generation from a KOH/CHCl 3 surrogate. For 5fdC synthesis, the protected 5-iodo-3',5'-( tert -butyl-dimethylsilyl)-2'-deoxycytidine was converted to the key intermediate, 5-formyl-3',5'-( tert -butyl-dimethylsilyl)-2'-deoxycytidine, through a vial-in-a-vial procedure (vial A: KOH, CHCl 3, and toluene; vial B: Pd 2 (dba) 3, xantphos, Bu 3 SnH [slow addition], toluene, and 65 °C) in a very good yield. An analogous approach was employed for the synthesis of the 5-methoxycarbonyl-2′-deoxycytidine precursor, with the carbonylative coupling conducted under modified conditions (vial A: KOH and CHCl 3 toluene; vial B: Pd 2 (dba) 3, xantphos, Et 3 N, and methanol, 55 °C). The KOH/CHCl 3 system provides significant safety advantages over gaseous CO allowing also synthetic efficiency at the microscale level. The 15 N 3 -labeled variants (4-amino-N1, N3- 15 N)-5fdC and (4-amino-, N1, N3- 15 N)-5cadC were synthesized with high isotopic purity and characterized by high-resolution mass (HRMS) and tandem mass (MS 2 ) spectrometry. These labeled standards were subsequently employed in liquid chromatography–isotope dilution tandem mass spectrometry (LC–ID–MS/MS) that is used for accurate quantification of endogenous 5fdC and 5cadC in DNA. This methodology provides the synthetic stable isotope “heavy”-labeled compounds needed and a robust analytical platform for investigating the formation and biological roles of these oxidatively generated epigenetic DNA lesions in the dysregulation of gene expression.