Beyond IC 50 : Reframing Microcystin Potency against Protein Phosphatase 2A by Defining Two-Step Irreversible Inhibition Kinetics
Abstract
] MC-RR binds PP2A with picomolar affinity despite lacking a highly reactive electrophile, demonstrating that high-affinity engagement is mechanistically separable from covalent inactivation. These results reveal that MC potency is driven by exceptionally tight and persistent PP2A binding rather than fast reactivity, providing a kinetic framework that links molecular structure to sustained phosphatase inactivation and real-world toxicity during harmful algal bloom exposure.
Fields of Study & Subjects
Aquatic Ecosystems and Phytoplankton Dynamics
Microtubule and mitosis dynamics
Protein Kinase Regulation and GTPase Signaling
Publication details
- Journal / source
- ACS Omega
- Publisher
- American Chemical Society (ACS)
- Volume
- 11
- Issue
- 19
- Pages
- 29202–29210
- Publication date
- 30 April 2026
- Language
- EN