Peptide-Based Dual-Readout Biosensor for the Detection of the Anti-Foot and Mouth Disease Virus Nonstructural Protein Antibodies
Abstract
Serodiagnosis of antibodies against the foot-and-mouth disease virus (FMDV) nonstructural protein 3ABC is essential for surveillance of infection and vaccination status. Here, we report a single-probe dual-readout peptide-based biosensor that combines electrochemical and fluorescence readouts for rapid and selective detection of anti-3ABC antibodies directly from serum. FMDV-inspired peptides, site-specifically labeled with 5(6)-carboxyfluorescein (FAM) or 7-methoxycoumarin-4-acetic acid, were immobilized on indium tin oxide electrodes via click chemistry. Antibody binding to the peptides produced a signal-off response in both modalities. FAM-probe electrodes exhibited superior performance, achieving detection limits of 0.38−1.14 (electrochemical) and 0.49−1.49 ng mL −1 (fluorescence) with
Fields of Study & Subjects
Animal Disease Management and Epidemiology
Viral Infections and Immunology Research
CRISPR and Genetic Engineering
Publication details
- Journal / source
- ACS Sensors
- Publisher
- American Chemical Society (ACS)
- Volume
- 11
- Issue
- 3
- Pages
- 1977–1989
- Publication date
- 16 February 2026
- Language
- EN