Rapid and Continuous Directed Evolution in Vibrio natriegens Utilizing an In Vivo Hypermutation System
Abstract
Genetic diversification serves as the foundation for in vivo molecular evolution. The fusion of a cytidine deaminase with T7 RNA polymerase (MutaT7) enables efficient diversification of DNA sequences downstream of the T7 promoter in model microbial hosts E. coli and S. cerevisiae . Vibrio natriegens, currently the fastest-growing nonpathogenic bacterium with a doubling time half that of E. coli, emerged as a promising alternative chassis for synthetic biology. Here, we report the establishment of MutaT7 in V. natriegens (dubbed VnMutaT7), achieving rapid evolution of the model protein TEM-1 to confer high-level ceftazidime resistance in just 36 h. This work establishes the potential of V. natriegens as an accelerated platform for protein engineering.
Fields of Study & Subjects
Vibrio bacteria research studies
Bacterial Genetics and Biotechnology
Invertebrate Immune Response Mechanisms
Publication details
- Journal / source
- ACS Synthetic Biology
- Publisher
- American Chemical Society (ACS)
- Volume
- 15
- Issue
- 7
- Pages
- 2875–2886
- Publication date
- 26 June 2026
- Language
- EN